The washouts of discarded bone marrow collection bags and filters are a very abundant source of hMSCs
C Capelli, A Salvadè, O Pedrini, V Barbui, E Gotti… - Cytotherapy, 2009 - Elsevier
Background aims Human multipotent mesenchymal stromal cells (hMSC) are considered
good candidates for a growing spectrum of cell therapies. We have validated a protocol that
makes use of the washouts of discarded collection sets, left over at the end of the filtration of
bone marrow (BM) explants performed for hematopoietic stem cell (HSC) transplantation.
Methods The method consists of direct plating of cells without density-gradient isolation
followed by two detachment steps and expansion in 5% human platelet lysate (hPL). Results …
good candidates for a growing spectrum of cell therapies. We have validated a protocol that
makes use of the washouts of discarded collection sets, left over at the end of the filtration of
bone marrow (BM) explants performed for hematopoietic stem cell (HSC) transplantation.
Methods The method consists of direct plating of cells without density-gradient isolation
followed by two detachment steps and expansion in 5% human platelet lysate (hPL). Results …
